Polyurethane (PUR)

Polyurethanes (CHEBI:60737) are polymers consisting of linkages between poly-alcohols (polyols) and di– or polycyanates. PUR is widely used synthetic polymers for the production of foams, insulation materials, textile coatings and paint to prevent corrosion. Ester-based PUR can be degraded by some microbes; but not ether-based forms. PUR is one of the few polymers, for which a number of microbial enzymes are known. They belong to the cutinases (EC, lipases (EC and carboxylesterases (EC and can act on amorphous and low crystalline ester-based PUR. These enzymes are often referred to as polyurethanases (PURases) (Wei et al., 2017; Danso et al., 2019). We further refer to Liu et al., 2021 for a detailed review of PUR-active microbial consrtia, enzymes and useful background information. Currently known and biochemically characterized active PUR-active hydrolases are listed below. Many PUR-active cutures of single and mixed species microbial communites have been reported recently. However, predicted and not characterized enzymes are not included. For PDB entries only one lead struture is included.

In case you are aware of PUR-active biochemically characterized enzymes that are not listed below, please contact the PAZY team.

Please cite the database by referring to: P.C.F. Buchholz, H.L. Zhang, P. Perez-Garcia, L-L. Nover, J. Chow, W. R. Streit, J. Pleiss (2022);Plastics degradation by hydrolytic enzymes: the Plastics-Active Enzymes Database - PAZy, https://doi.org/10.1002/prot.26325

Microbial host/enzyme/geneReferenceGenBank/ UniProtPDB entryNCBI BLAST
LCC, leaf compost metagenome, higly simliar to HRB29 locus GBD22443Schmidt, 2017G9BY574EBO and othersG9BY57.1
Thermobifida (Thermomonspora) fusca, TfCut_2 (Cut2-kw3) Schmidt, 2017E5BBQ3_THEFU4CG1CBY05530
Thermobifida (Thermomonspora) curvata, DSM43183, Tcur_1278 Wei, 2014D1A9G5 ACY96861.1
Thermobifida (Thermomonspora) curvata, DSM43183, Tcur0390Wei, 2014ACY95991.1 ACY95991.1
Rhodococcus equi TB-60, 45 kDa urethanase, purified, no gene identifiedAkutsu-Shigeno, 2006
Comamonas (Delftia) acidovorans TB-35, PudANakajima-Kambe, 1995 ; Nakajima-Kambe, 1997 ; Akutsu, 1998 ; Nomura, 1998; Shigeno-Akutsu, 1999BAA76305.1 BAA76305.1
Pseudomonas chloroaphis, lipase, PueA, PueBHoward, 1999; Ruiz, 1999; Howard, 2001; Howard, 2007; Stern, 2000AAD22743.1, AAF01331.1 AAD22743.1,
Pseudomonas fluorescens, esterase PulAHoward, 1998; Vega, 1999AAF66684.1 AAF66684.1
Pseudomonas protegens strain Pf-5 pueA and pueB Hung, 2016AAY92471.1; AAY92474.2 AAY92471.1,
Eukaryotic hosts
Pestalotiopsis microspora, lipase, 21 kDa hydrolase, purified, no gene identifiedRussel, 2011
Candida rugosa , lipase, Lip1-Lip5 isoenzymes, purifiedGautam, 2007

  • pur.txt
  • Last modified: 2022/03/02 14:09
  • by Golo Feuerriegel