Polyurethane (PUR)

Polyurethanes (CHEBI:60737) are polymers consisting of linkages between poly-alcohols (polyols) and di– or polycyanates. PUR is widely used synthetic polymers for the production of foams, insulation materials, textile coatings and paint to prevent corrosion. Ester-based PUR can be degraded by some microbes; but not ether-based forms. PUR is one of the few polymers, for which a number of microbial enzymes are known. They belong to the cutinases (EC, lipases (EC and carboxylesterases (EC and can act on amorphous and low crystalline ester-based PUR. These enzymes are often referred to as polyurethanases (PURases) (Wei et al., 2017; Danso et al., 2019; Masaki, 2022) . We further refer to Liu et al., 2021 for a detailed review of PUR-active microbial consrtia, enzymes and useful background information. Currently known and biochemically characterized active PUR-active hydrolases are listed below. Many PUR-active cutures of single and mixed species microbial communites have been reported recently. However, predicted and not characterized enzymes are not included. For PDB entries only one lead struture is included.

In case you are aware of PUR-active biochemically characterized enzymes that are not listed below, please contact the PAZY team.

Cite: Buchholz, P.C.F., Feuerriegel, G., Zhang, H., Perez-Garcia, P., Nover, L.-L., Chow, J., Streit, W.R. and Pleiss, J (2022);
Plastics degradation by hydrolytic enzymes: The Plastics-Active Enzymes Database - PAZy
(download link for reference manager)

Microbial host/enzyme/geneReferenceGenBank/ UniProtPDB entryNCBI BLAST
LCC, leaf compost metagenome, highly similar to HRB29 locus GBD22443Schmidt, 2017G9BY574EBO and othersG9BY57.1
Thermobifida (Thermomonspora) fusca, TfCut_2 (Cut2-kw3) Schmidt, 2017E5BBQ3_THEFU4CG1CBY05530
Thermobifida (Thermomonspora) curvata DSM43183, Tcur_1278Wei, 2014D1A9G5 ACY96861.1
Thermobifida (Thermomonspora) curvata DSM43183, Tcur0390Wei, 2014ACY95991.1 ACY95991.1
Lysinibacillus fusiformis Li, 2014; Jia, 2020KU353448
Rhodococcus equi TB-60, 45 kDa urethanase, purified, no gene identifiedAkutsu-Shigeno, 2006
Rhodococcus erythropolis CCM2595Kang, 2021;AGT90042.1
Rhodococcus erythropolis MP50Kang, 2021;AY026386
Sporosarcina ureae P17aKang, 2021;ARF18137.1
Pseudomonadota (synonym with Proteobacteria)
Comamonas (Delftia) acidovorans TB-35, PudANakajima-Kambe, 1995 ; Nakajima-Kambe, 1997 ; Akutsu, 1998 ; Nomura, 1998; Shigeno-Akutsu, 1999BAA76305.1 BAA76305.1
Pseudomonas chloroaphis, lipase, PueAHoward, 1999; Ruiz, 1999; Howard, 2001; Howard, 2007; Stern, 2000AAD22743.1 AAD22743.1
Pseudomonas chloroaphis, lipase, PueBHoward, 1999; Ruiz, 1999; Howard, 2001; Howard, 2007; Stern, 2000AAF01331.1 AAF01331.1
Pseudomonas fluorescens, esterase PulAHoward, 1998; Vega, 1999AAF66684.1 AAF66684.1
Pseudomonas protegens strain Pf-5, PueAHung, 2016AAY92471.1 AAY92471.1
Pseudomonas protegens strain Pf-5, PueBHung, 2016AAY92474.2 AAY92474.2
Agrobacterium tumefaciens d3, AmdAKang, 2021; Trott, 2001AAK28498.1 AAK28498.1
Klebsiella oxytoca 1686Kang, 2021;CP003218.1
Burkholderia phytofirmans DSM17436Kang, 2021;ACD16728.1 ACD16728.1
Microbacterium hydrocarbonoxydans Kang, 2021;GU116480 GU116480
Metagenome-derived, UMG-SP-1*Branson, 2022OP972509 OP972509
Metagenome-derived, UMG-SP-2*Branson, 2022OP972510 OP972510
Metagenome-derived, UMG-SP-3*Branson, 2022OP972511 OP972511
Eukaryotic hosts
Diutina (Candida) rugosa , lipase, Lip1-Lip5 isoenzymes, purifiedGautam, 2007P20261 P20261
Pestalotiopsis microspora, lipase, 21 kDa hydrolase, purified, no gene identifiedRussel, 2011
Candida parapsilosis NBRC 708 (CPUTNase)Masaki, 2020BBP47208 / XP_036664376 XP_036664376
Aspergillus oryzae Masaki, 2020Q12559 Q12559

* Degrades dicarbamates resulting from chemical pretreatment of polyether-polyurethane foam

  • pur.txt
  • Last modified: 2023/01/12 16:54
  • by Golo Feuerriegel