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Polyethylene (PE)

Polyethylene (CHEBI:60034) consists of long chain polymers of ethylene and it is produced as either high-density (HD-PE) or low-density polyethylene (LD-PE). PE is chemically synthesized by polymerization of ethane and is highly variable, since side chains can be obtained depending on the manufacturing process. Such modifications mainly have influence on crystallinity and molecular weight. The polymer is most frequently used in the packaging industry as one of the main packaging materials, and more than 100 million tons of PE are produced globally per year. Possible PE degradation has been affiliated with a surprisingly large number of bacterial genera. Among those were Gram-negative species affiliated with the genera Pseudomonas, Ralstonia, and Stenotrophomonas but also many Gram-positive taxa. In addition insect guts and their microbiota are explored as possible source for PE degradation. However no convincing biochemical data were generated that have identified defined enzymes and pathways.

Only four enzymes are kown to act in an oxidative but random manner on the polymer with very low efficiency (see below). In case you are aware of other PE-active and partially biochemically or strcturally characterized enzymes, please contact the PAZY team.

Cite: Buchholz, P.C.F., Feuerriegel, G., Zhang, H., Perez-Garcia, P., Nover, L.-L., Chow, J., Streit, W.R. and Pleiss, J (2022);
Plastics degradation by hydrolytic enzymes: The Plastics-Active Enzymes Database - PAZy
(download link for reference manager)

Host/enzyme/geneReferenceGenBank/ UniProtPDB entryNCBI BLAST
Eukarya
Galleria melonella, 'Demetra', arylphorin-like protein1 Sanlouis-Verdes, 2022XP_026756396.1 XP_026756396.1
Galleria melonella, 'Ceres', hexamerin1 Sanlouis-Verdes, 2022XP_026756459.1 XP_026756459.1
Bacteria
Rhodococcus opacus R7, LMC022 Zampolli, 2023AII11185 AII11185
Rhodococcus opacus R7, LMC032 Zampolli, 2023AII11221 AII11221

1 These two enzymes appear to result in an oxidative damage of the PE fiber and a release of small amounts of ketones ranging from C10 chain length to C22 and when icubated with PE having an average MW of 4000 Sanlouis-Verdes, 2022.

2 Treatment of PE with LMC02 and LMC03 resulted in release of mainly carboxylic acids (C8-C18) and small amounts of alkanes (C12-C30), 2-ketones (C11-C19) and alcohols (C10).


pe.txt · Last modified: 2024/03/24 11:09 by 127.0.0.1